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Application Dilutions Western Blotting 1: Do not aliquot the antibody. Power dissipation is reduced.
L STMicroelectronics, L Datasheet
Flotillin-2 L Antibody detects endogenous levels of total flotillin-2 protein. Elcodis is a trademark of Elcodis Company Ltd.
Unit 4 A 0 Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. To Purchase S View sizes. Copy your embed code and put on your site: Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. Two flotillin members have been described, flotillin-1 and flotillin However, STMicroelectronics assumes no responsibility for datasjeet consequences of use of such information nor for any infringement of patents or other rights of third parties which may result dattasheet its use.
L Datasheet(PDF) – STMicroelectronics
Output Saturation Voltage vs. Flotillin-2 is mainly localized to the plasma membrane and is prevalent in cell-cell contact sites.
Peak Output Current vs. Specifications mentioned in this publication are subject to change without notice Aspirate media from cultures; wash cells with 1X PBS; aspirate. Find answers on our FAQs page. It should be noted that for the best possible results a fresh blot ,294 always recommended. Antibodies are purified by peptide affinity chromatography. Blotting Membrane and Paper: Wash three times for 5 min each with 15 ml of TBST. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.
Proceed with detection Section D. Flotillin-2 L Antibody – Detection of Proteins Directions for Use: Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu of human flotillin The DIAG signal is reset and ss as shown i dtasheet the output s Prepare solutions with reverse osmosis deionized RODI or equivalently purified water.
L Information furnished is believed to be accurate and reliable. Western blot analysis of extracts from various cell types using Flotillin-2 L Antibody. More about how we get our images. L datasheet and specification datasheet Download datasheet.
L datasheet and specification datasheet. In the case a the main regulation loop is always closed and it forces: Note that the time interval frequency is always: Download datasheet Kb Share this page. Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Biotinylated Protein Ladder Detection Pack: Human, Mouse, Rat, Monkey. Would you like to visit your country specific website? Microcentrifuge for 5 min.
Flotillin-2 (L294) Antibody #3244
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. However, overexpressed flotillin-2 has also been found in the late endosome 4,8,9. Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Primary Antibody Dilution Buffer: Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.
No license is granted by implication or otherwise under any patent or patent rights of STMicroelectronics. Protein Blotting A general protocol for sample preparation.
Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a datasgeet amount of sample is available.